Product: Anti-ILP-2 pAb
Cat. No.: PC-540 (100 μg)
Synonyms:
Anti-Inhibitor of Apoptosis-Like Protein 2, anti-
IAP-Like Protein 2.
Background:
Apoptosis, or programmed cell death, is related
to many diseases, such as cancer. Apoptosis is
triggered by a variety of stimuli including
members in the TNF family and prevented by
the inhibitor of apoptosis (IAP) proteins. IAP
proteins form a conserved gene family including
IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind
to and inhibits specific proteases. A novel
member in the IAP protein family was recently
identified and designated ILP-2 for IAP-like
protein-2. ILP-2 has high homology to ILP-1, but
is encoded by a distinct gene that is solely
expressed in testis of tested normal human
tissues. ILP-2, unlike ILP-1, has no inhibitory
effect on Fas and TNF induced apoptosis, but
potently inhibits apoptosis induced by overexpression
of Bax or by co-expression of
caspase-9 with Apaf-1. ILP-2 interacts with the
processed caspase-9. These results suggest
that ILP-2 is a novel IAP family member with
restricted specificity for caspase-9.
Specificity:
Recognizes ILP-2.
Positive Controls:
HepG2 or MOLT4 cell lysates (~ 33 kDa band
can be detected by Western blot.
Species Reactivity:
Human. Other species not tested.
Host:
Rabbit
Immunogen:
Raised against a synthetic peptide
corresponding to amino acids near the aminoterminus
of human ILP-2 (Genbank accession
No. Q96P09).
Format:
100 μg of polyclonal antibody in PBS containing
0.02 sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 1 - 2 μg/mL.

Immunocytochemistry: Use at ~ 10 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of ILP-2 expression in
human HepG2 (lane 1) and MOLT4 (lane 2) cell
lysates with anti-ILP-2 pAb at 1 μg/mL.
Figure 2: Immunocytochemistry of ILP-2 in human
HepG2 cells with anti-ILP-2 pAb at 10 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any damage,
personal injury, or economic loss caused by this
product.