SUMO1 polyclonal antibody
产品名称： SUMO1 polyclonal antibody
英文名称： SUMO1 polyclonal antibody
产品编号： PAB2402
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of SUMO1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SUMO1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila.
Rathke C, Baarends WM, Jayaramaiah-Raja S, Bartkuhn M, Renkawitz R, Renkawitz-Pohl R.J Cell Sci. 2007 May 1;120(Pt 9):1689-700.
- 2.
- SUMO modification of the Ets-related transcription factor ERM inhibits its transcriptional activity.
Degerny C, Monte D, Beaudoin C, Jaffray E, Portois L, Hay RT, de Launoit Y, Baert JL.J Biol Chem. 2005 Jul 1;280(26):24330-8. Epub 2005 Apr 27.
- Applications
- Western Blot
- COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels) . Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels : cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels : cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with SUMO1 polyclonal antibody (Cat # PAB2402) . Bottom panels : cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with SUMO2/3 polyclonal antibody (Cat # PAB2408) . (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.
- Western Blot (Recombinant protein)
- The SUMO1 polyclonal antibody (Cat # PAB2402) is used in Western blot to detect GST-SUMO1 fusion protein.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SUMO1 polyclonal antibody (Cat # PAB2402) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 7341
- Protein Accession#:
- AAC50996;P63165
- Gene Name:
- SUMO1
- Gene Alias:
- DAP-1,GMP1,OFC10,PIC1,SENP2,SMT3,SMT3C,SMT3H3,SUMO-1,UBL1
- Gene Description:
- SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
- Omim ID:
- 601912
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq
- Other Designations:
- GAP modifying protein 1,SMT3 suppressor of mif two 3 homolog 1,sentrin,ubiquitin-like 1 (sentrin)