RPS6KB1 polyclonal antibody
产品名称： RPS6KB1 polyclonal antibody
英文名称： RPS6KB1 polyclonal antibody
产品编号： PAB1969
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of RPS6KB1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S411 of human RPS6KB1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- ERBB2, TBX2, RPS6KB1, and MYC alterations in breast tissues of BRCA1 and BRCA2 mutation carriers.
Adem C, Soderberg CL, Hafner K, Reynolds C, Slezak JM, Sinclair CS, Sellers TA, Schaid DJ, Couch F, Hartmann LC, Jenkins RB.Genes Chromosomes Cancer. 2004 Sep;41(1):1-11.
- 2.
- Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions.
Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S.Genome Res. 2004 Sep;14(9):1711-8.
- 3.
- Ribosomal p70S6K basal activity increases upon induction of differentiation of myelomonocytic leukemic cell lines HL60, AML14 and MPD.
Gomez-Cambronero J, Frye T, Baumann M.Leuk Res. 2004 Jul;28(7):755-62.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis of RPS6KB1 (arrow) using RPS6KB1 polyclonal antibody (Cat # PAB1969). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human breast cancer tissue tissue reacted with RPS6KB1 polyclonal antibody (Cat # PAB1969) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 6198
- Protein Accession#:
- NP_003152;P23443
- Gene Name:
- RPS6KB1
- Gene Alias:
- PS6K,S6K,S6K1,STK14A,p70(S6K)-alpha,p70-S6K,p70-alpha
- Gene Description:
- ribosomal protein S6 kinase, 70kDa, polypeptide 1
- Omim ID:
- 608938
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq
- Other Designations:
- p70 S6 kinase, alpha 1,p70 S6 kinase, alpha 2,ribosomal protein S6 kinase, 70kD, polypeptide 1,serine/threonine kinase 14 alpha