MAPK1 polyclonal antibody
产品名称: MAPK1 polyclonal antibody
英文名称: MAPK1 polyclonal antibody
产品编号: PAB9958
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against partial recombinant MAPK1.
- Immunogen:
- Recombinant GST fusion protein corresponding to C-terminus of human MAPK1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse, Rat
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:2000-1:10000)
Western Blot (1:1000-1:2000)
Immunoprecipitation (5.0 ug/mg)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis.
Asthagiri AR, Reinhart CA, Horwitz AF, Lauffenburger DA.J Cell Sci. 2000 Dec;113 Pt 24:4499-510.
- 2.
- Organization and regulation of mitogen-activated protein kinase signaling pathways.
Garrington TP, Johnson GL.Curr Opin Cell Biol. 1999 Apr;11(2):211-8.
- 3.
- The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579.
Hoffmann R, Baillie GS, MacKenzie SJ, Yarwood SJ, Houslay MD.EMBO J. 1999 Feb 15;18(4):893-903.
- Applications
- Western Blot (Cell lysate)
- Western blot using MAPK1 polyclonal antibody (Cat # PAB9958) shows detection of MAPK1 in several whole cell lysates : HeLa (lane 1), A-431 (lane 2), and NIH/3T3 (lane 3).
Detection occurs using a 1 : 1,000 dilution of the primary antibody followed by 1 : 4,000 dilution of HRP Goat-a-Rabbit with visualization via ECL.
Film exposure was approximately 1'.
- Entrez GeneID:
- 5594
- Protein Accession#:
- P28482;NP_002736
- Gene Name:
- MAPK1
- Gene Alias:
- ERK,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk
- Gene Description:
- mitogen-activated protein kinase 1
- Omim ID:
- 176948
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000174492,extracellular signal-regulated kinase 2,extracellular signal-regulated kinase-2,mitogen-activated protein kinase 2,protein tyrosine kinase ERK2
- Gene Pathway
- Acute myeloid leukemia
- Adherens junction
- Alzheimer's disease
- Axon guidance
- B cell receptor signaling pathway
- Bladder cancer
- Chemokine signaling pathway
- Chronic myeloid leukemia
- Colorectal cancer
- Dorso-ventral axis formation
- Endometrial cancer
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Fc gamma R-mediated phagocytosis
- Focal adhesion
- Gap junction
- Glioma
- GnRH signaling pathway
- Insulin signaling pathway
- Long-term depression
- Long-term potentiation
- MAPK signaling pathway
- Melanogenesis
- Melanoma
- mTOR signaling pathway
- Natural killer cell mediated cytotoxicity
- Neurotrophin signaling pathway
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prion diseases
- Prostate cancer
- Regulation of actin cytoskeleton
- Renal cell carcinoma
- T cell receptor signaling pathway
- TGF-beta signaling pathway
- Thyroid cancer
- Toll-like receptor signaling pathway
- Type II diabetes mellitus
- Vascular smooth muscle contraction
- VEGF signaling pathway